circular plasmids (New England Biolabs)
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New England Biolabs
circular plasmids
Circular Plasmids, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 287 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/circular+plasmids/MfeI/pmc12962856-64-2-7
Average 96 stars, based on 287 article reviews
Circular Plasmids, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 287 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/circular+plasmids/MfeI/pmc12962856-64-2-7
Average 96 stars, based on 287 article reviews
circular plasmids - by Bioz Stars,
2026-09
96/100 stars
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Purification:Article Title: RNA localization to nuclear speckles follows splicing logic. Article Snippet: .. For linearization, Article Title: Genome-wide identification of functional enhancers and their potential roles in pig breeding Article Snippet: The vector backbone was linearized using PCR (95 °C for 5 min; then 25 cycles of 95 °C for 20 s, 57 °C for 20 s and 72 °C for 4 min; Forward primer: 5'-CGT CGT GTA GGG AAA GAG TGT-3' and Reverse primer: 5'-GCA CAC GTC TGA ACT CCA GTC-3'). .. Further, Article Title: RNA localization to nuclear speckles follows splicing logic Article Snippet: .. For linearization, Article Title: RNA localization to nuclear speckles follows splicing logic Article Snippet: .. For linearization, Polymerase Chain Reaction:Article Title: RNA localization to nuclear speckles follows splicing logic. Article Snippet: .. For linearization, Article Title: RNA localization to nuclear speckles follows splicing logic Article Snippet: .. For linearization, Article Title: RNA localization to nuclear speckles follows splicing logic Article Snippet: .. For linearization, Agarose Gel Electrophoresis:Article Title: RNA localization to nuclear speckles follows splicing logic. Article Snippet: .. For linearization, Article Title: Genome-wide identification of functional enhancers and their potential roles in pig breeding Article Snippet: The vector backbone was linearized using PCR (95 °C for 5 min; then 25 cycles of 95 °C for 20 s, 57 °C for 20 s and 72 °C for 4 min; Forward primer: 5'-CGT CGT GTA GGG AAA GAG TGT-3' and Reverse primer: 5'-GCA CAC GTC TGA ACT CCA GTC-3'). .. Further, Gel Extraction:Article Title: Genome-wide identification of functional enhancers and their potential roles in pig breeding Article Snippet: The vector backbone was linearized using PCR (95 °C for 5 min; then 25 cycles of 95 °C for 20 s, 57 °C for 20 s and 72 °C for 4 min; Forward primer: 5'-CGT CGT GTA GGG AAA GAG TGT-3' and Reverse primer: 5'-GCA CAC GTC TGA ACT CCA GTC-3'). .. Further, Functional Assay:Article Title: Genome-wide enhancer identification by massively parallel reporter assay in Arabidopsis. Article Snippet: Yongjun Tan, Xiaohao Yan, Jialei Sun, Jing Wan, Xinxin Li, Yingzhang Huang, Li Li, Longjian Niu and Chunhui Hou State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650201, China, Department of Biology, Southern University of Science and Technology, Shenzhen 518055, China, Department of Bioinformatics, Huazhong Agricultural University, Wuhan 430070, China, School of Public Health and Emergency Management, Southern University of Science and Technology, Shenzhen 518055, China, and Shenzhen Key Laboratory of Cardiovascular Health and Precision Medicine, Southern University of Science and Technology, Shenzhen 518055, China Plasmid Preparation:Article Title: A Conserved Motif in Tetrahymena thermophila Telomerase Reverse Transcriptase Is Proximal to the RNA Template and Is Essential for Boundary Definition Article Snippet: PCR Mutagenesis—Plasmids containing the T. thermophila RBD fused to an N-terminal His tag were PCR-mutagenized using custom DNA primers. .. Linear plasmid amplicons were treated with T4 polynucledotide kinase (NEB) and T4 DNA ligase to generate |